The Ability of Conducting Polyaniline/Multiwall Carbon Nanotubes/Gold Nanoparticle Nanocomposite for Cholesterol Measurment

Cholesterol oxidase (ChOx) has been immobilized on polyaniline (PANI) and multiwall carbon nanotubes (MWCNTs) glad nanoparticle (AuNPs) nanocomposite film by using physical absorption method. Electrochemical behavior of PANI/MWCNTs/AuNPS films has been studied using cyclic voltammetry (CV) and PANI/MWCNTs/AuNPs/ChOx enzyme film Ampromertic (AMP) techniques, respectively. In this work, the amperometric response of a Pencil graphite disk electrode (PGDE) modified with ChOx/MWCNTs/PANI was studied in the presence of AuNPs. The response of AMP in constant potential 0.7 V, in buffer phosphate solution (PBS) 0.1M (pH 7.5 and T 40 C◦) was applied to measuring of various cholesterol concentrations. The optimized condition for the determination of cholesterol shows linear range of 0.01-6.01 mM, sensivivty 204 nA mM -1 a response time of about 2s. The biosensor showed only 20% loss in its initial response after 60 days when stored at 4 C◦.

Keywords:

Gold Nanoparticle , Polyaniline , Multiwall Carbon Nanotubes , Cholesterol Oxidase , Immobilization , Cholesterol