Synergistic effects of serotonin and D-lys3- GHRP-6 on food intakes in food deprived male rats

Introduction: D-Lys3 -GHRP-6 or serotonin exerts an inhibitory effect on food intakes. In the present study, we aimed to investigate the effect of serotonin on food intakes in D-Lys3- GHRP-6 treated food deprived male rats. Materials and methods: Twenty food deprived male rats in four groups (in each group n=5) received third cerebral ventricular injections of saline, 10μg serotonin, 20nmol D-Lys3 -GHRP-6 or combination of serotonin/D-Lys3-GHRP-6 in a volume of 3μl respectively. Mean one hour food intakes were measured. Data were analyzed by one-way analysis of variance ANOVA with post hoc Tukey’s test. Results: In all cases P<0.05 was considered to be statistically significant. Serotonin, D-Lys3- GHRP-6 or combination of serotonin/D-Lys3- GHRP-6 decreased significantly mean food intakes compared to saline. A significant decrease was observed on mean food intakes between the combination of serotonin/D-Lys3- GHRP-6 group and serotonin or D-Lys3- GHRP-6 groups. A significant decrease was not observed on food intakes between serotonin and D-Lys3- GHRP-6 groups. Serotonin and D-Lys3-GHRP-6 may exert synergistic inhibitory effects on food intakes in rats. Conclusion: Decrease of the ghrelin pathway activity may have a role in the mediating of the inhibitory effects of serotonin on food intakes

Keywords:

Serotonin , D-Lys3- GHRP-6 , Food intakes