Evaluation of cytotoxic properties of Aqueous and alcoholic extracts of liquorice plant (Glycyrihizaglabra L) on DU-145 cell line

Introduction: Prostate cancer is the second most common cancer in men after skin cancer. Throughout the years, medicinal plants, and in some cases the only treatment were considered. Several medicinal plants have been identified to have anti-cancer properties and also plants as a source of antioxidants and phenolic compounds are considered. Licorice plant with the scientific name Glycyrrhizaglabra L belongs to the family Fabaceae, with three species of perennial herbaceous plants in Iran. Root extract is widely used around the world in the medical, food, drug and other industries. In this study, the cytotoxic effect of Aqueous and alcoholic extracts of licorice root of human prostate cancer (DU145) was determined by MTT assay. Materials and methods: Licorice root through Agriculture and Natural Resources Research Center of Isfahan was prepared. Then the Aqueous and alcoholic extracts of the plant were made. DU-145 cell line RPMI-1640 medium containing 10% bovine serum in an incubator with 5% CO2 and the Aqueous and alcoholic extracts of cultured under different concentrations of 24, 48 and 72 hours was incubated. MTT assay to determine the survival rate of the cells in the presence and absence of the extracts was determined by ELISA absorbance read at 540 nm. Data were investigated by using SPSS software (* = P-value < 0.05, N=12). Results: The results show a decrease in the survival rate of the cells was determined by MTT assay. Conclusion: The results of this study, it states that aqueous extract of licorice plant may have some cytotoxic effects, because of terpene compounds in licorice.

Keywords:

Cytotoxic , Licorice plant , Cell's line