Isolation, identification and serotyping uropathogenic escherchia colisiderophore manufacturer of hospital patients and health care centers in Bandare Anzali

Introduction: From the viewpoint of Microbiology, urinary tract infection, there is a time, Pathogenic microorganisms are in the urine, bladder and be found in the prostate, or kidney. In the most cases, growth of more than 105 Microorganisms per ml of the sample middle of the urine is indicative of infection. But in cases of marked, number of 104-102 bacteria in each ml of urine also indicative of infection. Symptoms of dysuria, emergency and urination with bacterial infection a sizeable not associated acute bladder syndrome called. The aim of this study was to determine the cases of urinary tract infection. Identification of strain UPEC, serotyping and antimicrobial resistance, reviews of the siderophore production and hemolysin and their relations with the ability to create an infection, in the strain Escherichia coli isolated from these was patients. Materilas and methods: In order to separating and identification of the bacterial agent UPEC a urinary tract infection and determination of age release and gender people suffering in a prospective study number of 200 sample was taken from the middle of the urine random sampling method and simple was analysis, test of sensitivity to various antibiotics with the method of Kirby-Bayer namely put the disc on the agar containing 21 isolation UPEC isolated from these patients by age range 86-1 year and serotyping agglutination test method performed on the Lam and the hemolysin production on sheep blood agar with Balad 5% studied. Alsosiderophore production in isolation, are also examined. All results with the use of the SPSS software analytics. Results: Majority of isolated UPEC urinary tract pathogens belonging to 0157, O126, O1, O20, O25, O44, O15, O55, O64, O26, O114 was serotypes. Majority of the isolated antibiotics, ampicillin, cotrimoxazole and cefixime and cephalexin most of the resistance and to the amikacin and gentamicin and ciprofloxacin, tetracycline, maximum sensitivity. Conclusion: This study showed serotype O25 maximum number of isolated cases will be included and so many other serotypes included O157, O26, O64, O15, O126, O114, O55, O1, O44, O20

Keywords:

Urinary tract infection , UPEC Escherichia coli , Serotype , Antimicrobial susceptibility , Siderophore