Separation and Determination of Doxepin in water samplesby Air-agitated Dispersive Liquid-liquid microextractionmethod prior to UV-Vis Spectrophotometer

the present research, a new and fast microextraction procedure which is a combination of air agitated dispersive liquid-liquid microextraction using low density organic solvent and directly suspended droplet microextraction method was used for the extraction of trace amounts of doxepin from real samples prior to UV-Vis spectrophotometer. The parameters influencing the extraction efficiencies including kind ofthe extracting solvent, the volume of the aqueous sample solution (donor phase), the volume of extraction solvent (acceptor phase), the number of air injected, the effects of pH and salt were optimized. Under the optimal conditions, the obtained enrichment factor is above 80. By plotting peak areas of the standard solutions versus various concentrations of the analyte, calibration curve is obtained which show that thelinear ranges of 0.001-2 μg mL-1 with correlation coefficient (r) of 0.994. The precision of the method (RSD %, n=5) was calculated less than 4.5 and the limit of detection (LOD) was 0.003 μg mL-1. The proposed microextraction method was used for the extraction of the analyte from environmental water samples and the calculated relative recoveries were all above than % 85

Keywords:

Two liquid-phase microextration (LLME) , Doxepin , UV-Vis spectrophotometer , Wateranalysis