Production and fatty acid characterization of DH1025a doubled haploid Camelina sativa line

Camelina sativa L. as an oilseed plant, belongs to Brassicaceae family, has been indicatedin several experiments that need very little water and resistant to chilling injury than otherplant oils, especially canola. This Experiment was conducted at Zagros Bioidea Co. Laband Research Field of Campus ofAgriculture and Natural Resources. In this researchBlaine Greek and Calena cultivars have been crossed. The F1 generation anthers werecultured for doubled haploid lines production. Buds 1–3 mm were removed from theplant and selected for culture. The anthers were isolated and cultured in NLN mediumwith 120 g/l sucrose. Then cultured anthers were incubated in 25C and dark conditions.After 4 weeks in order to regeneration, the embryos were sub-cultured to B5 mediumsupplemented with 10 g/l sucrose. The incubation conditions were 25C and 16hphotoperiod. The regenerated plants were selfed and seed was produced. Then multipliedthe seed and tested in dryland farming conditions. Among the studied lines, the DH1025shoed the best compatibility and performance (1500 kg/ha average seed yield and oilcontent of about 27.9%). The main fatty acids identified in this line were as α-linolenicacid (ALA) (29.70%), linoliec acid (LA) (21.03), Oleic acid (16.41%) and eicosenoicacid (14.09). The details of this profile have been reported in this paper

Keywords:

Camelina sativa , oilseed , dryland , oil profile , fatty acid