Ashraf Haj Hosseini - Department of Science and Food Engineering, Islamic Azad University, Science and Research Branch, Tehran
Afshin Mahmoudzadeh - Member of the faculty of science,Department of Biology, Roudehen Branch, Islamic Azad Uniersity
Anousheh Sharifan - Assistant Professor of Department of Science and Food Engineering, Islamic Azad University, Science and Research Branch, Tehran

In this present study, biosurfactant producing microorganism seudomonasa eruginosa, was isolated. The bio surfactant production was studied using a Minimal Salt Medium with crude oil (1 %) as the hydrocarbon. The optimization study was carried out using various carbon, nitrogen sources, pH, temperature, hydrocarbons, and trace elements. The effect of dry cell biomass, biosurfactant production, emulsification activity and surface tension were studied. The glycerol and sodium nitrate was the best carbon and nitrogen source studied with the isolate. The environmental factors such as pH 7 and temperature 30oC were found to be optimum for the biosurfactant production. Among the hydrocarbon tested the crude oil and n-hexadecane showed statistically on par results

Pseudomonas aeruginosa, Biosurfactant, Emulsification activity, Surface Tension