Effects of missense R84Q mutation on human Pyrroline-5-carboxylate synthase enzyme properties, an in-silico analysis
Publish place: Journal of Applied Biotechnology Reports، Vol: 1، Issue: 1
Publish Year: 1392
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_JABR-1-1_002
تاریخ نمایه سازی: 29 آذر 1395
Abstract:
Mammalian Δ-(1)-Pyrroline-5-carboxylate synthase (P5CS) enzyme catalyzes the coupled phosphorylation and reduction-conversion of glutamate to Δ-(1)-pyrroline-5- carboxylate (P5C), a critical step in the proline, ornithine, citrulline and arginine biosynthesis. In plants and mammals, P5CS consists of two separate enzymatic domains:N-terminal γ-glutamyl kinase (γ-GK) and C-terminal γ-glutamyl phosphate reductase (γ–GPR). Hyperammonemia has been reported as a new inborn disorder, with a range of clinical symptoms which is associated with a reduced synthesis ofproline, ornithine, citruline and arginine. A missense mutation, R84Q, which altersthe conserved residue in γ-GK domain, is responsible for this disorder. In this study using in-silico approaches as a new bioinformatics method, sequence analysis was performed and the tertiary structure of γ-GK domain of human P5CS, which includesthe R84Q missense mutation, was predicted and the mutation effects on structural and functional features of P5CS enzyme were analyzed. Our analysis showed that this substitution has an affect on the molecular surface accessibility and total energy of the modeled structure. We conclude that this mutation results in a reduced activity of P5CS enzyme and an impaired synthesis of these amino acids.
Authors
Maryam Zare
Department of Biology, Payame Noor University, Avaj, Iran
Faranak Hadi
Department of Biology, Faculty of Science, Lorestan University, Khoramabad, Iran.
Zarrin Minuchehr
Industrial and Environmental Biotechnology Department, National Institute of Genetic Engineering & Biotechnology (NIGEB), Tehran, Iran.
Jafar Amani
Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.