Enhancing iron oxidation efficiency by a native strain of Acidithiobacillus ferrooxidans via response surface methodology, and characterization of proteins involved in metal resistance by proteomic approach

Publish Year: 1392
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_JABR-1-1_005

تاریخ نمایه سازی: 29 آذر 1395

Abstract:

The effects of different factors on growth and bio-oxidation efficiency of a native strain of Acidithiobacillus ferrooxidans have been evaluated by the utilization ofresponse surface methodology, RSM. Medium pH and iron concentration were found to be the most significant factors while temperature and ammonia concentrations hadthe least weight within the ranges investigated. Optimum operational conditions for maximizing Fe2+ oxidation were found to be 31 oC, 7 g/Liron concentration, 4.5 g/Ltotal ammonium salt concentration and medium pH 1.85. Maximum recovery of 98% of Zinc is the main outcome of results as observed at 7 g/Lof Fe2+, under optimized experimental conditions. The response of a bacterial strain to metalstoxicity also studied. The isolate showed good resistance to most of the toxic metals. The proteomics approach was used to identify the differentially expressed proteinsunder heavy metal stress. Four of the differentially expressed proteins were identified as major outer membrane protein of A. ferrooxidans, ribulose large bisphosphate carboxylase subunit, and holo synthase

Authors

Fahimeh Nemati

Islamic Azad University, Pharmaceutical Sciences Branch, Tehran, Iran

Daryoush Arabian

Department of Bioscience and Biotechnology,Malek Ashtar University of Technology, Tehran, Iran

Rasool Khalilzadeh

Department of Bioscience and Biotechnology,Malek Ashtar University of Technology, Tehran, Iran

Fazin Abbaspour Aghdam

Faculty of Chemical Engineering, Sahand University of Technology, Tabriz, Iran