Determination of T-2 Mycotoxin in Fusarium strains by HPLC with fluorescence detector
Publish place: Journal of Applied Biotechnology Reports، Vol: 1، Issue: 1
Publish Year: 1392
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_JABR-1-1_007
تاریخ نمایه سازی: 29 آذر 1395
Abstract:
T-2 toxin is the most poisonous trichothecene produced by Fusarium species especially F.sporotrichioides. T-2 toxin is a biological contaminant in a number of agriculturalcommodities that can cause severe diseases among humans and animals and even lead to death. The aim of the current study is the analysis of T-2 mycotoxin in Fusarium species by high-performance liquid chromatography (HPLC) combined with florescence detection and derivatization with 1-antroylnitrile (1-AN). Totally,11Fusariumisolates and reference strains were studied. The isolates were tested forthe T-2 toxin production after growing on rice substrate followed by using specific Multisep 225 Trich Clean up columns purification. In this study, T-2 toxin production was ranged from 197.05ug/kg to 8503.07ug/kg. This is the first study of T-2 toxin analysis by HPLC-F in Iran
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Authors
Reza Kachuei
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Sassan Rezaie
Department of Medical Parasitology and Mycology,Faculty of Public Health, Tehran University of Medial Sciences, Iran
Mohammad Hossein Yadegari
Department of Medical Mycology, Faculty of Medicine Science, Tarbiat Modares University, Tehran, Iran
Naser Safaie
Department of Plant Pathology ,Faculty of Agriculture ,Tarbiat Modares University ,Tehran , Iran