Improvement of Thermal Stability of DFPase by In silico Methods
Publish place: Journal of Applied Biotechnology Reports، Vol: 1، Issue: 4
Publish Year: 1393
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_JABR-1-4_004
تاریخ نمایه سازی: 29 آذر 1395
Abstract:
Efficiency of enzymes which are used in industrial or environmental applications is highly dependent on their thermal stability. In this study, the stability of DFPasehas been evaluated after introducing disulfide bonds to the structure. The results obtained from a series of protein design software were subjected to moleculardynamics simulation at different temperature to test the performance of such combinatorial procedure. Amount several designs, mutation M5 showed desirablethermostability via molecular dynamics simulation and normal mode analysis. As it clearly depicted, such in silico structural investigations would be resulted in reducing the numerous choices of experimental options as it was undergone a series of computational evaluation previously.
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Authors
Morteza Mirzaei
Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Ali Mohammad Latifi
Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Rahim Jafari
Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran