Effect of Genotype, Explant Type and 2,4-D on Cell Dedifferentiation and Callus Induction in Cumin (Cuminum cyminum L.) Medicinal Plant

Publish Year: 1394
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_JABR-2-3_001

تاریخ نمایه سازی: 29 آذر 1395

Abstract:

Cumin (Cuminum cyminum L.), a member of the Apiaceae family, is one of the most important medicinal plants in the world. An experiment was conducted for theevaluation of callus induction optimization in cumin accessions from four different regions: Shahdad, Koohbanan, Badrood, and Afghanistan. A factorial experimentbased on completely randomized design was conducted on MS medium supplemented with different concentrations of 2,4-D (0, 0.5, 1 and 2 mg/L) plus 0.1mg/L Kinetin in different explants (Root, Shoot, Leaf, Embryo and Seed) of cumin accessions. In this experiment the evaluated traits were days to callus induction, callus induction percentage, and callus growth rate. Statistical analysis showed thatseed (as the latest) and root (as the earliest) explants require 54 and 11 days for the initiation of callus induction, respectively. Results showed that accession, explant,accession × explant and 2,4-D × explant interactions had statistically significant effects (P<0.01) on callus induction percentage and callus growth rate. Furthermore, 2,4-D had a significant effect on callus induction percentage. According tothe results of this study, in plants of some regions the root explant is an appropriate explant for large production of callus in some accessions of Cuminum cyminum. Also, Koohbananian accession produced callus in shorter time and the Afghanian one produced high number of callus.

Keywords:

2 , 4-D , Callus Growth Rate , Days to Callus Induction , Seed Explant

Authors

Jahad Soorni

Department of Agronomy and Plant Breeding, Razi University, Kermanshah, Iran

Danial Kahrizi

Department of Agronomy and Plant Breeding, Razi University, Kermanshah, Iran