Identification of Volatile Organic Compounds from Trichoderma virens (6011) by GC-MS and Separation of a Bioactive Compound via Nanotechnology

Publish Year: 1395
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_IJE-29-10_016

تاریخ نمایه سازی: 12 دی 1395

Abstract:

Fungal volatile organic compounds (VOCs) have the potential of being used as biocontrol agents forbiotechnological applications in agriculture, industry and medicine. In this research, different VOCsfrom secondary metabolites of biocontrol fungus Trichoderma virens (6011) were separated using nhexane,n-butanol and methanol solvents and identified by gas chromatography–mass spectrometry(GC-MS) device. According to mass spectra library searching, more than 200 volatile compounds (withspectral match factor higher than 80%) such as alkanes, alkenes, alcohols, organic acids, aromaticcompounds, aldehyde, etheric, esteric, phenolic, kenotic derivatives and, sulfur and nitrogencompounds, have been detected. Some of the VOCs such as dibutyl phthalate (DBP) had antifungalactivity. The antifungal effect of DBP as a case study was checked and confirmed in in-vitroconditions. DBP as a bioactive compound was separated from secondary metabolites using MolecularlyImprinted Polymers (MIPs) as a solid sorbent. Two kinds of the MIPs were synthesized via bulkpolymerization and precipitation polymerization. Nanoporous MIPs for DBP, with binding capacityca.462 mg.g-1 and the specific surface area 479m2.g-1 were synthesized via bulk polymerization methodwhile the synthesized MIPs via precipitation technique had the binding capacity ca.830 mg.g-1 withspecific surface area 690 m2.g-1. The synthesized MIPs were evaluated by scan electron microscopy(SEM) device and Brunner Emmett-Teller (BET) analysis. Results showed that, the MIPsnanotechnology can be suggested as a suitable alternative method for separation of the chemical toxins.This study introduces a simple method under laboratory conditions to separate the bioactive compoundsfrom fungal secondary metabolites.

Keywords:

Brunner Emmett-tellerGas Chromatography–mass SpectrometryMolecularly Imprinted PolymersSeparationTrichoderma virensVolatile Organic Compounds

Authors

M Shahiri Tabarestani

Department of Plant Protection, Faculty of crop Production, Gorgan University of Agricultural Sciences & Natural Resources, Gorgan, IRAN

K Rahnama

Department of Plant Protection, Faculty of crop Production, Gorgan University of Agricultural Sciences & Natural Resources, Gorgan, IRAN

M Jahanshani

Nanotechnology Research Institute, Babol Noshirvani University of Technology, Mazandaran, Iran

S Nasrollanejad

Department of Plant Protection, Faculty of crop Production, Gorgan University of Agricultural Sciences & Natural Resources, Gorgan, IRAN