Allelic polymorphism of K-casein, β-Lactoglobulin and leptin genes and their association with milk production traits in Iranian Holstein cattle

Publish Year: 1392
نوع سند: مقاله ژورنالی
زبان: Persian
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شناسه ملی سند علمی:

JR_JCMR-5-2_002

تاریخ نمایه سازی: 7 بهمن 1395

Abstract:

The purpose of this study was to investigate the polymorphism of K-casein (K-CN), β-Lactoglobulin (B-LG) and leptin (LP) genes in Iranian Holstein cattle by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. DNA was extracted from blood samples of 139 cows using a modified phenol chloroform method. Association between K-CN, B-LG and LP genes’ polymorphism with milk production traits were investigated using mixed procedure of SAS software. The frequencies of AA, AB and BB genotypes for K-CN (0.72, 0.18 and 0.10), B-LG (0.43, 0.28 and 0.29) and LP (0.24, 0.63 and 0.13) were also calculated. Statistical results revealed a significant association between AA and BB genotypes of the K-CN gene with milk production and milk protein percentage, respectively. Also, BB genotype of the B-LG gene and AA genotype of the LP gene showed a significant association with protein percentage and milk production (P<0.05), respectively. Therefore, it is feasible to improve milk production traits in these herds using the studied genes

Authors

Yahya Mohammadi

Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

Ali Asghar Aslaminejad

Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

MohammadReza Nassiri

Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

Ali Esmailizadeh Koshkoieh

Department of Animal Science, Faculty of Agriculture, university of Shahid Bahonar , Kerman, Iran