Development of anew electrochemical aptasensor for Sensing of epirubicin

Anthracycline antibiotics are the most effective anticancer treatments ever developed, used in cancer chemotherapy (1). Among them, epirubicin (EPI) has been widely used to treat varietyof cancers but clinical utilization of EPI is confined, because of dose-related cardiotoxicity and bone marrow suppression (2, 3). Therefore, it is highly desirable, to promote a highly sensitive and selective method for detection of EPI for clinical diagnosis and attaining the best possibletherapeutic effect. Aptamers are artificial single-stranded oligonucleotides that selected through SELEX (4). Upon binding of aptamer with high affinity to targets, tertiary structures were formed. In this manuscript, a novel label free electrochemical aptasensor was fabricated based on the magneticNanocomposite for detection of EPI. The immobilization of the magnetic Nanocomposite on theSPE increased the surface area and conductibility of the aptasensor, which amplified the electrochemical signals. Interaction of EPI with the aptamer and the properties of the fabricated aptasensor were examined with cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS).

Keywords:

Aptamer , Epirubicin , Electrodeposited gold nanoparticles , Carbon Screen-printed electrode