Hydroquinone diphosphate/Ag+ as an enzymatic substrate for alkaline phosphatase catalyzed silver deposition
Publish place: 11th Annual Electrochemistry Seminar of Iran
Publish Year: 1394
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
ELECTROCHEMISTRY011_102
تاریخ نمایه سازی: 5 بهمن 1395
Abstract:
Signal amplification by enzyme labels such as alkaline phosphatase (AP) used in affinity assays (immuno or DNA based assays) is ausual strategy to get high sensitive detection methodologies. An advantageouscombination of an enzyme substrate Hydroquinone diphosphate (HQDP) with silver ions (Ag+) insolution is presented in this work as a new enzymatic substrate for AP. This enzyme catalyzes the dephosphorylation of HQDP producing hydroquinone, a reducing agent for silver ions present in solution. Thus by mixing AP, HQDP and Ag+, metallic silver is deposited where the enzymatic reaction takesplace. A qualitative assay is presented in nitrocellulose membranes to demonstrate this mechanism. The affinity reaction between biotin and streptavidinlabelled with AP is also followed by the electrochemical detection of silver deposited on the surface of Screen Printed Carbon Electrodes. Anodic stripping voltammetry of enzymatically deposited silver is showed as a sensitive detection technique for biosensing applications.
Authors
Marta María Pereira Da Silva Nevesa
DropSens, S.L; Ed.CEEI, Parque Tecnológico de Asturias, 33428 Llanera, Asturias, Spain
María Begoña González Garcíaa
DropSens, S.L; Ed.CEEI, Parque Tecnológico de Asturias, 33428 Llanera, Asturias, Spain
David Hernández Santosa
DropSens, S.L; Ed.CEEI, Parque Tecnológico de Asturias, 33428 Llanera, Asturias, Spain
Pablo Fanjul-Bolado
DropSens, S.L; Ed.CEEI, Parque Tecnológico de Asturias, 33428 Llanera, Asturias, Spain