Hydrazine as an important example of NH2 containing compounds is a strong reducing agent widely used in various field, including fuel cells, industrial, agricultural, pharmacological,military and aerospace applications [1]. Of note, It is highly toxic and easily absorbed by oral, breathing routes of exposure or even introduced to the skin, which mostly affect the lungs, kidney, liver and central nervous system of living organisms. Moreover, It may cause liver andkidney damages, also irreversible deterioration of nervous system when it absorbs through skin. Accordingly, a sensitive and fast determination in low concentrations in various media is becoming more and more important. Electrochemical methods appear to be very promising sincethey ensure reasonably good analytical performance characteristics with essentially no need for expensive and complicated instrumentation [2]. Carbon paste is one of the most popularmaterials to prepare solid-state electrodes, with high applicability in electrochemistry research. The ease and speed of preparation and obtaining a new reproducible surface, low residual current, porous surface and low cost are advantages of carbon paste electrodes (CPEs) over all other solid electrodes [3]. Recently, the application of the carbon paste electrodes modified(MCPEs) with various materials exhibits considerable improvements in electrochemical behavior of many important compounds. As is well-known, nanostructure materials have been used to fabricate a new type of chemically modified electrodes (CMEs) with rapid electron transferkinetic [4]. In this work, a ferrocene-derivative compound, 2,7-bis (ferrocenyl ethynyl) fluoren- 9-one (2,7-BFE), was synthesized and used to construct a modified-graphene oxide nano-sheets paste electrode. The electro-oxidation of hydrazine at the surface of the modified electrode was studied using electrochemical approaches. Under the optimized conditions, the square wave voltammetric peak current of hydrazine increased linearly with hydrazine concentrations in the range of 2.2×10-7 to 3.0×10-4 M and a detection limit of 9.8×10-8 M was obtained for hydrazine. Finally this modified electrode was used for determination of hydrazine in some water samples