Electrochemical determination of anti-HIV drug Nevirapine with a nanocomposite modified graphite electrode by gold nanoparticles and poly(methylene blue) on multi wall carbon nanotube substrate

In this work the graphite electrode was modified by electrodepositing of Au nanoparticles on electropolymerized methylene blue (p-MB) and multi-walled carbon nanotubes (MWCNTs) fordetermination of nevirapine (NEV), an anti-HIV drug. The electrochemical oxidation of NEV was studied by voltammetric techniques. NEV is a non-nucleoside reverse transcriptase inhibitor(NNRTI) of HIV, that causes acquired immunodeficiency syndrome (AIDS). The HIV reversetranscriptase is an important antiviraltarget for the chemotherapy of AIDS because of its keyrolein virus replication[1]. Electrochemical method is an alternative approach with the advantages of simple, quick and low cost. The electropolymerization of MB from aqueous solution and its electrocatalytic activity towards biomolecules and different inorganic compounds have already been reported [2-5].The effects of pH and scan rates on the oxidation of NEVwere studied. Differential pulse voltammetry (DPV) technique was employed for the determination of NEV and the obtained results confirmed that the oxidation peak currents increased linearly with NEV concentrations in the ranges of 0.2-46 μM with detection limit of 50 nM. Finally, the Au NPs/p-MB/MWCNTs/GE was successfully used for the determination of NEV in serum and pharmaceutical samples.

Keywords:

Nevirapine , anti-HIV drug , poly (methylene blue) , MWCNTs , modified graphite electrode