Sahar Amiri - Department of Chemistry, Faculty of Science, Rasht Branch, Islamic Azad University, Rasht, Iran
Sara Sandoni - Department of Chemistry, Faculty of Science, Rasht Branch, Islamic Azad University, Rasht, Iran
Mohammad Ali Bagherinia - Department of Chemistry, Faculty of Science, Lahijan Branch, Islamic Azad University, Lahijan, Iran

Ion-selective electrodes (ISE) are useful devices for determining the different cationic and anionic species because of high speed, low cost, simple preparation, a wide dynamic range andfinally no requirement for the sample pretreatment [1]. In addition of using ISEs for direct determination of the investigated species they can be also used for indirect determination of other species [2]. Excess ammonia in the breeding pool may cause massive death of fish and otheraquatic organisms due to eutrophication and depletion of the dissolved oxygen [3]. Furthermore,bacteria can convert ammonia into nitrite, which is a potent carcinogen [4]. Therefore, measuring ammonia content in water is important for aquaculture, drinking water and environmentalmonitoring. Traditional methods for measuring ammonia content were based on Nessler’s reagent colorimetry, indophenol, Ophthaldialdehyde (OPA) and N-acetyl-cysteine (NAC) reagent, and titration. These methods are commonly cost and need the primary preparation steps.In this research, a new nanosensor were prepared by incorporating sytosine amino acid as a ionophore with together a modified magnetic nano particles into a plasticized poly(vinyl chloride) matrix. The general procedure used to prepare the PVC membrane was mixingthoroughly optimised amounts of 30 mg of powdered PVC, 60 mg plasticizer DBP, 5 mg additive KTpClPB, 5 mg magnetic nano particles Fe3O4 modified and 2 ml of dry freshly distilled THF into a glass dish of 2 cm diameter. To this solution for the fabrication of theammonium-selective membran 5 mg of sytosine as an ionophore was added and mixed very well. The solvent was evaporated at the room temperature. After 2 h the polymer membranecould be easily removed from the plate. Then the transparent PVC membrane was taken up fromthe plate and attached to the end of a glass tube with a diameter of 1 cm and height of 5 cm by means of PVC-THF viscose solution. The NH4+-selective electrodes were each time backfilled with NH4Cl internal filling solution of 0.01 mol dm-3 conditioned. Then the electrodes wereconditioned in 0.01 mol dm-3 solution of MgCl for 48 h. Calibration plots with Nernstian slopes (59.6 ± 0.8 mV/decade) for ammonium ion wereobserved over a linear range of concentration (1.0 × 10-6 to 1.0 × 10-1 mol dm-3, at 25 oC). This electrode revealed a lower limit of detection of 3.9 × 10-7. It has a short response time about 15 second. The influence of membrane composition, the pH of the test solution, the interfering ions on the electrode performance and the effect of temperature on the electrode response are investigating.

Ammonium selective, PVC membrane, Nano particles, Potentiometric method