Stripping Voltametric Determination of Vitamin B6 Using Modified Glassy Carbon Electrode with β-Cyclodextrin Functionalised Graphene NanoSheets

Vitamin B6 (VB6) helps the body to make antibodies, transmit signals in the brain, maintain normal nerve function, make haemoglobin and keep blood sugar in normal ranges. β-Cyclodextrin (β-CD) is a very stable oligosaccharide that is composed of seven glucose units linked with each other by α-(1,4)-glycosidic linkage. The CDs have a truncated cone-resembled shape with a hollow cavity. The depth of the hollow cavity is 0.78 nm for all three types (α, βand γ) of CDs. Thus, a variety of guests can be encapsulated into the cavity via the host-guest interaction in aqueous conditions and even in the solid state. On the other hand Graphene with a unique two-dimensional structure, is predicted to be an excellent electrode material for energystorage and conversion system due to its large surface area, high electrical conductivity, flexibility, and mechanical strength. Therefore β-Cyclodextrin functionalised graphene is able simultaneously possessing unique properties of graphene and β-Cyclodextrin. In this studyfabricated modified glassy carbon electrode with β-yclodextrin functionalised graphene (GC/Gr/β-CD) and applied for electrochemical measurement of VB6. Cyclic voltammetry was employed to evaluate the electrochemical performance of modified electrode and compare its electrochemical performance with modified glassy carbon with graphene (GC/Gr) and bare electrode. As can be seen of fig (1) GC/Gr/β-CD is capable in VB6 oxidation than other twoelectrodes. After optimization all effective parameter in measurment such as pH, instrumental parameter we obtain calibration curve for VB6 determination By methods of differential pulse voltammetry (DPV), square wave voltammetry (SWV) and linear sweep voltammetry (LSV) thatshown in Fig (2) to (4). In Table (1) show the results obtain from calibration curves and Compared with other works. To examine the predominant type of mass transport a study was carried out using cyclic voltammetry on scan rate effect and the result shows electrooxidation reaction is an adsorption controlled process.

Keywords:

graphene , cyclodextrin , modified electrode , stripping voltammetry , vitamin B6