Seyid Javad Musevi - Analytical Chemistry Research Lab., Faculty of Basic Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran
Habib Razmi - Analytical Chemistry Research Lab., Faculty of Basic Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran
Rahim Mohammad-Rezaei - Analytical Chemistry Research Lab., Faculty of Basic Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran

Graphene, a new class of 2D carbon nanomaterials with one atom thickness, has attracted considerable attention in recent years (1). Eggshell membrane is cheap, green and easilyachieved biopolymers which have been used frequently in analytical science (2). In the present study, reduced graphene oxide is doped efficiently into the soluble eggshell membrane protein(RGO-SEP) and the resultant biocomposite is introduced as a novel and effective nano-sorbent in SPE for the preconcentration of Hg2+. In order to increase the sensitivity and detection limit of the developed method, anodic stripping voltammetry (ASV) technique was used as a detection method. The scheme of the preconcentration and determination process was shown in scheme 1.Here Hg2+ was preconcentrated in two steps: first in SPE process which Hg2+ preconcentrated according to enrichment factor; second in ASV technique which Hg2+ accumulates on the electrode surface. Thus the developed method is very sensitive, cost effective, and green in comparison with other reported techniques.

Anodic Stripping Voltammetry; Eggshell Membrane; Mercury Analysis