Evaluation and optimization of the factors affecting the performance of glucose biosensor

The fast and accurate determination of glucose has profound applications since glucose concentration is a crucial indicator in many diseases, such as diabetes, endocrine metabolicdisorder. In recent years, many efforts have been made to develop reliable glucose biosensors using electrochemical [1], chemiluminescence [2], or other methods [3]. Among all the methods, enzyme-involved electrochemical glucose biosensor has been intensively studied because of itssimplicity, high selectivity and relative low cost [4-6]. Recently, zinc oxide (ZnO) nanostructures have drawn many attentions in the fabrication of biosensors with many advantages, including nontoxicity, biological compatibility, fast electron transfer rates and easy preparation. ZnO thinfilm was prepared via electrodeposition method and its surface morphology was studied by scanning electron microscopy (SEM). Charactrization of GOX adsorption on ZnO thin film surface was performed using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques. The impedimetric results were explained for glucose detection.

Keywords:

Glucose oxidase , ZnO nanostructure , Glucose biosensor