Asma Khoobi - Department of Analytical Chemistry, Faculty of Chemistry, University of Kashan, Kashan P.O. Box 87317-51167,Islamic Republic of Iran
Nasrin Soltani - Department of Chemistry, Payame Noor University (PNU), Tehran 19395-4697, Islamic Republic of Iran
Mostafa Aghaei - Department of Chemistry, Payame Noor University (PNU), Tehran 19395-4697, Islamic Republic of Iran

Paracetamol (PCT) is commonly applied to control mild-to-moderate pain or to reduce opioid exposure as part of multimodal analgesia, and is the only compound recommended to treat feverin neonates [1]. PCT has low toxicity when used at the recommended doses. Nevertheless, the ease with which the general public can access this compound makes it frequently associated with overdoses, in this situation causing serious or even fatal hepatic damage [2,3]. So it is veryimportant to create a sensitive, fast, accurate and simple method for PCT detection. In the present work, an electrochemical nanosensor for PCT was fabricated based on iron oxide nanoparticles by modification of carbon paste electrode surface. At first, iron oxide nanoparticles weresynthesized and then used for preparation of an iron oxide nanoparticles modified carbon paste electrode (ION/CPE). The characterization of iron oxide nanoparticles was carried out throughdifferent methods such as X-ray diffraction (XRD), scanning electron microscopy (SEM) andvoltammetry. The oxidative behavior of PCT was investigated by different voltammetric techniques at the surface of ION/CPE. Other novelty of the wok is application of multivariateoptimization methodology for sensitive detection of PCT. Using the proposed method, theION/CPE showed an excellent electrocatalytic activity for the oxidation of PCT, and accelerated electron transfer between the electrode and PCT. Under the optimal experimental conditions, the differential pulse peak current was linear with the concentration of PCT in the range of 0.01-250.0 μM with a detection limit of 2.0 nM. The proposed electrode revealed good stability and reproducibility. Moreover, the proposed method was successfully applied for the determination of PCT in real biological samples.

Iron Oxide Nanoparticles, Paracetamol, Multivariate Optimization, Electrochemical Methods