Pt nanoparticles /Fe/Al -Layered Double Hydroxide Modified Glassy Carbon for Sensitive Determination of Warfarin

Layered double hydroxides (LDHs) also called anionic clays have been recently attracted because of their potential in the field of catalysis, adsorption and nanocomposite [1]. The LDHscomposition can be expressed as [Ma(II)1-x Mb(III)x (OH)2]x+ (Ax/n)n-.mH2O formula, where M(II) and M(III) can be most divalent and trivalent metal ions and A– is any type of anions [2]. Due to desirable properties of LDHs nanoparticles, they can serve as an effective support for metalnanoparticles. In recent years, noble metal nanoparticles (NPs) such as Pt, Au, Pd NPs have widely been considered due to their excellent properties such as stability, biocompatibility, low cytotoxicity and catalytic properties. Warfarin, 4‐hydroxycoumarin, is widely used asanticoagulant drug to treat and prevent thromboembolism [3]. Warfarin has a narrow therapeutic index having a propensity for many drug–drug interactions which require conscientious monitoring of its concentrations to maintain it within therapeutic range [4]. Therefore, in clinicaltreatment, it is useful to develop a fast and sensitive method with simple sample preparation and determination steps for warfarin analysis. In this work, a sensitive warfarin sensor has beenfabricated by electrodepositing of Pt nanoparticles on Fe Al-layered double hydroxide modifiedglassy carbon. Study of layer formation on the electrode surface followed by electrochemical impedance spectroscopy and cyclic voltammetry using redox couples Fe(CN)63-/4- species. The nanocomposite was characterized by field emission scanning electron microscopy, fourier transform infrared and energy dispersive X-Ray spectroscopy. The fabricated sensor exhibits excellent electrochemical catalytic activities toward the oxidation of warfarin. The oxidationpeak current was proportional to the concentration of warfarin from 0.1 to 400.0 μmol L-1with a detection limit of 0.03 μmol L-1 at signal to noise ratio of 3. The capability of the proposed sensor for determination of warfarin was examined by the standard addition method in real samples.

Keywords:

Warfarin , Pt nanoparticles/ Fe/Al -Layered double hydroxide modified glassy carbon , Electrochemical sensor , Electrocatalytic determination