Naser jafarzadeh - Department of Medical Physics, Tarbiat Modares University, Tehran, I.R. Iran.
mohammad javad rasaee - Department of Medical Biotechnology , Tarbiat Modares University, Tehran, I.R. Iran.
rasoul malekfar - Atomic & Molecular group, Department of Physics, Tarbiat Modares University, Tehran, I.R. Iran
afshin mohsenifar - Research & Development Department, Nanozino Co., Tehran, I.R. Iran.

Here, we have a developed surface-enhanced Raman spectroscopy substrate composed of antibody-conjugated silver nanoparticles as a functional nanoprobe. In this study, we synthesized silver nanoparticles with the para-mercaptobenzoic acid (pMBA) linker that binds to antibody Herceptin Here, we analyzed the binding of Herceptin to silver nanoparticles by Fourier Tranform Infrared (FT-IR). Our results clearly showed that a spherical shape of silver nanoparticles with a diameter of 55 nm. Furthermore, our SERS probe apparently indicated that the intensity of antibody-conjugated silver nanoparticles as a SERS sensitive probe is increased by an enhancement factor of 155.

Silver nanoparticles, antibody conjugation, Raman scattering, spectroscopy, surface enhanced, SERS