The review of Baculoviruse Expression Vectors system
Publish place: کنفرانس بین المللی محیط زیست و منابع طبیعی
Publish Year: 1394
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
IENC01_021
تاریخ نمایه سازی: 5 بهمن 1395
Abstract:
Baculoviruses are insect-specific viruses widely used for the production of recombinant proteins, vaccines and viral pesticides. It has become a common expression system for both basic research and large-scale commercial applications. The baculoviruses expression system is based on the replacement of non-essential, viral gene, termed polyhedrin, with a gene of interest. The insertion of foreign DNA at the polyhedrin locus within the viral genome results in incorporation of the foreign DNA into progeny virus particles and subsequent high-level expression of the recombinant protein within eukaryotic insect cell lines. A main factor to the popularity of insect cell expression is the ability of insect cells to produce relatively large quantities eukaryotic proteins in a relatively short period of time.In this review, we focus on the structure of baculoviruses and applications of baculovirus-insect cell expression system.
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Authors
Neda Hashemi
Department of Food Science and Technology, Azad University of Sabzevar
Sayed Ali Mortazavi
Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad
Samane Gazerani
Department of Food Science and Technology, Azad University of Sabzevar
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