Effect of surfactant concentration and lipid: oil ratio on the particlesize and stability of beta carotene nano lipid carrier

The objectives of the present study are to prepare and produce betacarroten -contained NLCs, andsurvey the effect of surfactant concentration and lipid: oil ratio on the particle size and stability ofbetacarotene NLC. The Hot-High shear Homogenizer (Hot-HSH) technique was applied to produceNLC dispersions and the optimum formulation determined with different circumstances change,such as surfactant concentration (2, 3, 4 and 6% w/v) and lipid:oil ratio (Compritol ATO 888/ octyloctanoate) in 2:1,4:1and 10:1. Various testes such as particle size, morphology and stability carriedout to determine physicochemical properties of formulations. NLCs prepared at 3% (w/v)poloxamer 407 concentration and at 10:1 ratio of compritol (solid lipid) to octyl octanoate (liquidoil) containing 2% (w/v) beta carotene concentration showed significantly lower particle size andnarrow particle size distribution (73 nm and span = 0.684, respectively). The results showed that anoptimum concentration of 3% of Poloxamer 407 was sufficient to cover the surface of nanoparticlesefficiently and use 10:1 ratio of solid lipid liquid oil showed significantly lower particle sizecompare to other ratios . The production of betacarroten-contained NLCs was an efficacious methodin terms of encapsulation, nanoscale particle size decrease, stability within time and other expectedspecifications

Keywords:

nano lipid carrier , octyl octanoate , ploxamer , stability , beta carotene