Synthesis and characterization of thermosensitive nanocomposite gels based on magnetic graphene oxide and poly (N-isopropyl acryl amide) and investigation on their application in doxorubicin drug delivery as an anticancer drug

To improve intracellular Doxorubicin (DOX) delivery, DOX-encapsulated nanocomposite gel based on magnetic graphene oxide incorporated with thermo-responsive drug release are developed. The biomedical applications of magnetic graphene oxide, including drug delivery, have grown in the recent few years. Magnetic graphene oxide has been surveyed for biomedical applications due to its unique properties: two dimensional planer structure, large surface, chemical and mechanical stability, and also good biocompatibility. So, these features are beneficial for the design of advanced drug delivery systems. In addition, Poly (N-isopropylacrylamide) is a temperature responsive polymer, which shows a lower critical solution temperature at 32◦ in aqueous solutions. Nanogels based on this temperature sensitive polymer swell at a low temperature and collapse at a high one. Sensitive nanocomposite gels based on magnetic graphene oxide, are so important because of being cheap and having surface more than volume and they are synthesized for drug delivery systems. In this paper, we present an overview of recent advances in this field of research. Also, we describe the synthesis of thermosensitive nanocomposite gels based on magnetic graphene oxide, nanocarrier, and poly (N-isopropylacrylamide), and survey their toxicity and biocompatibility. Besides, drug delivery is done based on controlling mechanisms, which is including targeting and stimulation with pH, thermal and so on. In this project, morphology and size and structure of nanocomposite gels are determined via SEM and DLS and TEM. Also, the amounts of drug releasing are surveyed via UV spectra, by time. The magnetic property of magnetic graphene oxide is indicated by VSM. In vitro feature shows that the DOX replaced nanogels endocytosed by Hela cells which can release DOX in acidic organelles. As a result, it is shown that the best intracellular drug delivery system is prepared by DOX loaded nanogel.

Keywords:

Nanocomposite gel , Magnetic graphene oxide , Controlled drug delivery system , Poly (N-isopropylacrylamide)