Effects of different synthesis parameters on the Controlled Growth Factor Delivery from the Smart Temperature Sensitive Polymer

The aim of this research was to synthesis a temperature sensitive polymer and to investigate its capability to deliver the bioactive growth factors. Different formulation of Poly (N-isopropylacrylamide) (PNIPAM) was successfully synthesized by free radical polymerization technique. The formation of PNIPAM nanoparticles and its optimal formulation was confirmed by different analytical methods. The presence of particles with spherical grains in the range of 50–100 nm was observed in all samples. Optimized polymeric nanoparticles had particle sizes less than 100 nm and LCST of 30-32 °C. The biological evaluation of PNIPAM nanoparticles performed according to the ISO 10993-5 cytotoxicity standard. The particles up to a concentration of 3 mg/ml did not show any toxicity on MC3T3-E1 mouse osteoblast cell lines. Temperature dependent release kinetic of the basic fibroblast growth factor (bFGF) was closely studied via human bFGF elisa kit. The cumulative release profile study revealed that around 37 °C the synthesized smart nanoparticles maintain sustained release of bFGF over the first 72 hr