Investigation the effect of surface on volume of culture media and value of inoculant on microbial cellulose biopolymer production

Microbial cellulose nanofiber produced by some microorganism via internal cells metabolism and due to unique properties they have many industrial applications. According to the importance of fermenter in increasing the yield of microorganism, in this research cellulosic fiber produced in various plates Acetobacter Xylinum local strain. The fibers were removed from culture medium surface after 10 days and investigated after purification and drying. FTIR, weighting, water absorption and SEM for assessing the morphology structure were used in order to compare the samples. Obtained results confirm the cellulosic chemical structure and it indicated that the amount of cellulose production increase by increasing the surface to volume ratio. In addition, figures of fiber’s diameter taken by SEM were not showing any noticeable changes in different plates. Their diameters are between 30-90 nm. Therefore, the obtained results of this research represent that producing of cellulose is increased 1.5 times and 2 times by changing the ratio of surface to volume and increasing the inoculant respectively