PLGA/NMP injectable systems containing temporary nanocomposite microparticles

In this research, potential of a poly (lactic-co-glycolic) acid (PLGA)/ N-methyl-2-pyrrolidone (NMP) based injectable system containing gelatin/alginate/nanohydroxyapatite as a temporary cell encapsulation devise) for bone tissue engineering applications have been investigated. The microparticles were prepared by injection of aqueous solution containing specified amount of gelatin, alginate and nanohydroxyapatite into CaCl2 solutions. PLGA based systems with different contents of nanocomposite microparticles were characterized using X-ray Diffraction (XRD), Thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and Ultraviolet–visible spectroscopy (UV/Vis). The results show that after 48 hours, more than 80% of NMP released from the samples. In addition, porosity calculation showed that as time passes, porosity increases and finally reaches to 70-73%. Morphological studies showed that a 50wt% micro-particles based system is the optimum samples for osteoblast in tissue engineering