A New Practical Approach to Study Nano-Micro Pore Structure of Electrospun Nano-Microfibrous Scaffolds by Assembling CdSe/ZnS Quantum Dots

Specific inter-fiber pore structures are required to provide the needed biological and biophysical functions for fibrous scaffolds as these architectures are critical to cell infiltrationand in-grows performance. However, the key challenging on evaluating 3D pore structure of fibrous scaffolds for betterunderstanding the capability of different structures for biological application is not well investigated. This paper reports a fast, accurate, non-destructive, and comprehensiveapproach to study the inter-fiber pore structure of electrospun Nano-Microfiber scaffolds. Quantum dots (QDs) with strong,symmetric fluorescence emission, and photochemically stable properties were incorporated into nanofibers. Confocal laserscanning microscopy (CLSM) was employed to capture the 3D pore structure of Electrospun Polycaprolactone (PCL) scaffolds. Internal pore structure of different electrospun fibrous scaffolds has been investigated by this approach and the results were compared with existing method