Bardia Moradi - Horticultural Science PhD student of Islamic Azad University, Science and Research Branch, Tehran
Bahram Delfan - Lorestan University of Medical Sciences
Peyman Hashemi - Lorestan University · Department of Chemistry
Amir Mousavi - National Institute of Genetic Engineering and Biotechnology (NIGEB)

The use of bacterium Agrobacterium rhizogenes for hairy roots induction in medicinal plants species of due to increased amounts of metabolites, high proliferation rate of interest is located. We investigate the optimization of hairy root cultures Hyoscyamus reticulatus plant showed that strains of bacteria and explant type effectively transformed explants on impulse. An MSU strain has the greatest impact on transformation and the second effective strains were A7 and D7. Hypocotyl and cotyledon explants of creating transgenic plants showed more effective.