Study of Escherichia coli strains 091, 026 Antibiotic resistances in diarrhea cases in Chaharmahal & Bakhtiary province

The aim of study is more learning to the prevalence and antimicrobial susceptibility of O91, O26 serotypes for faecal colonization in ChaharMahal & Bakhtiary Province. O91, O26 serotypes cause to bloody diarrhea, Hemorrhagic colitis (HC) and Hemolytic and uremic syndrome (HUS). E. coli O26 is the most frequently isolated non-O157 Shiga-toxigenic E. coli (STEC) associated with human clinical illness. Used Drugs, Cefuroxime, Ceftazidime, Doxycycline, Piperacillin/Tazobactam, Imipenem and Ciprofloxacin. 284 samples from patients with diarrhea were collected and cultured on MacConkey agar. Indole test, methyl red, VogesProskauer was performed. Kirby-Bauer test using 6 beta Lactam antibiotics. PCR primers for E. coli O26: wzxF, wzxR and E-coli O91: P50A, P50B. Data was analyzed by SPSS 19. 164 (58%) strains of Escherichia coli were isolated from 284 collected clinical faecal samples. nine O26 strain (5.5%) and twenty-one O91 strain (13%) was isolated. Antibiotic Resistance to Cefuroxime, Ceftazidime, Doxycycline, Piperacillin/Tazobactam, Imipenem and Ciprofloxacin respectively 54.3%, 48.8%, 91.5%, 29.3%, 29% and 48.8%. There was significant relationship between Doxycycline Resistance and outpatients (54%) p<0.05. Doxycycline resistance in age group 2-15 years was higher than others age groups. Piperacillin/Tazobactam by 7% in age group 16-24 and Imipenem by 8.5% resistance in age group 25-50 have a lowest antibiotic resistance p<0.05.

Keywords:

Escherichia coli O91 , O26. Antibiotic resistance