ARMIN TAHMASBI RAD - Nanobiomaterials Laboratory, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran
MANA NOVITIN - Nanobiomaterials Laboratory, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran
EHSAN SADEGHILAN DEHIKORD - Nanobiomaterials Laboratory, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, IranMEHRAN SOLATI-HASHJIN Nanobiomaterials Laboratory, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran
SHAHAB FAGHIHr - Tissue Engineering and Biomaterials Division, National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. box. ۱۴۱۵۵-۶۳۴۳, Tehran, Iran

The integration of hard tissue to implant surface is one of the most important properties to optimize the stability and efficiency of tissue-implant interface. To meet this conditions the formation of appropriate interface of tissue and biomaterial is necessary to occur tissue regeneration and optimal interaction of tissue and implant. Titanium alloys are widely used to manufacture orthopedic implants. One approach to enhance the integration of tissue to implant is using biocompatible coatings on implant surface. Nano-Hydroxyapatite is known as the most bioactive material to coat on implants surface. Many coating techniques have been employed for the preparation of HA coatings; however Electrophoretic Deposition (EPD) process exhibits some advantages over other alternative processes. In this project each sample rubbed with sand papers with different degrees of roughness from 60 to 1200. The final polishing was done using soft polishing machine. Remained wear and waste products on the surface of samples were removed using sonic bath machine. Due to increase the surface roughness, samples are etched by a solution of Nitric Acid and Hydrochloric Acid. Due to alkaline treatment samples were submerged in a NaOH solution. Hydroxyapatite powder prepared via the precipitate method. Then Hydroxyapatite suspension was prepared in ethanol solution. The EPD process was accomplished to coat HA on each sample. Samples were sintered and the X-ray diffraction (XRD), EDS and Fourier transform infrared spectroscopy (FTIR) techniques were used to characterize structure and composition of the powders and coating. To study the cellular attachment and cell morphology, MG63 cells were cultured on the surface of samples. The results demonstrated that homogeneous nano-HA coating on samples can be obtained with those 2 Armin Tahmasbi Radet al.post-treatments and coated samples showed better bioactivity and cellular attachment. The scanning electron microscope (SEM) images illustrated that the coated substrates showed better cell morphology and better cellular response than non-coated pure titanium. Hence EPD can be used as an accurate and flexible method and thin film of nano-HA coating that makes the implant much more bioactive and biocompatible.

Nanohydroxyapatite; Titanium; Coating; Electrophoresis; biological assessments, MG63 Cells