Shirvan Salaminia - Department of Surgery, Yasuj University of Medical Sciences, Yasuj, Iran
Saman Nikeghbalian - Shiraz Organ Transplantation Center, Namazee Hospital, Shiraz University of Medical Sciences, Shiraz, Iran
Farzaneh Dehghani - Department of Anatomy and Histology, Morphometric Stereology Research Laboratory, Shiraz University of Medical Sciences, Shiraz, Iran
Babak Sabet - Department of General Surgery, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Background: The surgical management of liver injuries remains a great challenge for the traumatologists andgeneral surgeons. We hypothesized that administration of 17 β-estradiol, a female sex hormone, improveshepatocellular healing after liver trauma.Methods: In an experimental model, 60 rats were divided into six subgroups: A (male control), B (male andestradiol), C (castrated male and estradiol), D (female control), F (female and estradiol), and G(oopherectomized female). After inducing liver trauma, estradiol subgroups received 3 doses of intravenous 17β-estradiol (1 mg/kg) every 8 hours. 2 weeks post trauma, animals were sacrificed and hepatocellularregeneration was measured with the help of stereologic parameters of regeneration. Hepatocellular healing wascompared between previous left lobe samples and the new post-traumatic right lobe samples.Results: Stereological parameters of rats receiving 17 b-estradiol after trauma was much better regarding meanangiogenesis point counting and volume density, compared with non-receiver groups after 2 weeks of trauma(P < 0.005). There was no significant difference for hepatocyte nucleus, hepatocyte point counting and volumedensity between estradiol receiver and non-receiver groups. In a comparison between subgroups, female sex hadthe same effect as giving estradiol. Oopherectomized female rats had more fibrogenesis but less angiogenesis(P < 0.005). Fibrogenesis was more in groups that were estradiol non-receiver (P < 0.005). In an explicitcomparison of control females and males, estradiol infused males and females, and castrated male oroopherectomized female groups showed that stereological parameters of hepatocyte and hepatocyte nucleus werelower in female subgroups, but angiogenesis was better for female groups except for oopherectomized females.Conclusions: This study did support the administration of exogenic female hormone as an approach toaugment the angiogenesis as a good index of regeneration for traumatic liver in rats.© 2015 Tehran University of Medical Sciences. All rights reserved.Citation: Salaminia Sh, Nikeghbalian S, Dehghani F, Sabet B, Mafi AA, Malek Hosseini SA, et al. TheEffects of Sex Hormones on Liver Regeneration after Liver Trauma in Animal Model. Acad J Surg, 2015;2(1-2): 2-6.

Liver diseases, Liver regeneration, Hepatocytes, Gonadal steroid hormones, Estradiol,Hormone replacement therapy, Models, Animal, Rats