Survival of Lactobacillus Acidophilus as Probiotic Bacteria using Chitosan Nanoparticles
Publish Year: 1396
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJE-30-4_001
تاریخ نمایه سازی: 6 شهریور 1396
Abstract:
Chitosan was used for nanoencapsulation of Lactobacillus acidophilus as probiotic bacteria. In vitro experiments were conducted with the objective of investigating the survival of the bacterial cells in gastro-intestinal conditions. The results demonstrated that the size of chitosan nanoparticles noticeablyincreased by increasing chitosan concentration from 0.05 to 0.5 g/mL. Encapsulation of the cells caused a decrease at the leakage of probiotic bacteria when compared to free bacteria. However, thenumber of probiotic cells reduced from 3.27 to 3.23 log CFU/mL in gestural acid condition in contrast to free cells which they approximately dropped from 3.3 to 3 log CFU/mL after 120 min. Goodprobiotic viability and stability was also obtained by nanocapsulation of Lactobacillus acidophilus in intestinal juice. At this condition, the initial capsulated cells numbers were 3.28 log CFU/mL whichafter placing in biliary salt condition for 120 min, it reached to 3.23 log CFU/mL. On the other hand, the free bacteria cells reduced from 3.3 to 2.97 log CFU/mL in intestinal environment. Overall,nanocapsulation of probiotic bacteria plays a pivotal role in enhancing the viability and survival of them against gastro- intestinal environmental conditions
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Authors
p Ebrahimnejad
Department of Pharmaceutics, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran . Pharmaceutical Sciences Research Center, Mazandaran University of Medical Sciences, Sari, Iran
m Khavarpour
Department of Chemical Engineering, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
s Khalili
Department of Food Science and Technology, Sabzevar Branch, Islamic Azad University, Sabzevar, Iran