Bacteriological study of caudal fin rot in brood stocks of Salmo trutta caspius using PCR/RFLP from the propagation and breeding center of Shahid Bahonar, Kelardasht

Objective: The aim of the present study was to investigate the bacterial infection in caudal fin rot in brood stocks of Salmo trutta caspius using PCR/RFLP. Method and materials: In order to study caudal fin rot with emphasis on Aeromonas hydrophila and Pseudomonas fluorescens in Salmo trutta caspius from the Salmonids Propagation and Breeding Center of Shahid Bahonar of Kelardasht region, one hundred and eighty brood stocks having fin damage symptoms were chosen. Two bacterial samples from each fish were cultured on Aeromonas and Pseudomonas specific media. Similarly, in second stage of the study, bacterial samples were taken from thirty brood stocks. For isolation and biochemical diagnosis of the genera Aeromonas and Pseudormonas, the samples were studied using PCR amplification (using 16S rDNA genes of the genus Pseudomonas and 16S-23S rDNA intergenic spacer of the genus Aeromonas) and restriction analysis by four restriction enzymes for each genus. Results and conclusion: Restriction analysis of Aeromonas samples with Hin6I, Csp6I, TaqI, and TasI revealed three samples were different from others while restriction analysis of Pseudomonas samples with AluI, HinfI, RsaI, and Tru1I showed at least five species or biovars.

Keywords:

Aeromonas , Pseudomonas , Salmo trutta caspius , PCR/RFLP