Nasim Eini - Department of Agricultural Biotechnology, Payame Noor University, Tehran, I.R. of IRAN
Samira Shahbazi - Nuclear Science and Technology Research Institute (NSTRI), Atomic Energy organization of IRAN (AEOI), I.R. of IRAN
Razieh Sarabadani - Department of Agricultural Biotechnology, Payame Noor University, Tehran, I.R. of IRAN
Hamed Askari - Nuclear Science and Technology Research Institute (NSTRI), Atomic Energy organization of IRAN (AEOI), I.R. of IRAN

Trichoderma secreted several cellulases and glucanases to lysis the Pythium mycelia in mycoparasite process. Enhancement in these enzyme production in T. viride by gamma radiation is a useful mutation method, and resulted in increasing antagonistic potential against an important plant pathogen Pythium ultimum. In this study, proteomics analysis and cellulase assay were used to indicate the role of endo-glucanase and exoglucanase enzymes in bio-control. The results of cellulase enzyme activity of T. viride mutant isolates and its wild type strain after 48 h incubation at 180 rpm and 28 ˚C with different substrate (Avicel, Carboxy methyl cellulose and Bacterial cellulose) are shown the enhancement in the enzyme activity values of the mutant isolates. The specific exo-glucanase enzyme activity in Tv M21 mutant isolate approximately 2-2.5 times more than its wild type strain and also secreted 3 times more endoglucanase. This superior mutant showed up to 65% growth inhabitation against P. ultimum in dual culture test (5 times more than the wild type) and sharper spots belong to endo-glucanase, exoglucanase and β-glucosidase presented in SDS-Page and 2D Electrophoresis of this mutant. Overall, induced mutation by gamma irradiation could be a useful method to access such superior mutants and TvM21 could be a successful BCAs candidate for plan disease management programs of Pythium inoculated soils.

Trichoderma viride; γ-irradiation; SDS-Page; 2D Electrophoresis;Cellulase