Molecular analysis of Trichomonas gallinae by using of Fe-hydrogenase gene by PCR reaction in Columbidae birds

Trichomonas gallinae is flagellated protozoan that caused avian trichomonosis. This organism is widespread in clombiforms. In most cases, infection with T. gallinae in birds can be asymptomatic. Diagnosing of T. gallinae depends traditionally on direct microscopic observation of motile protozoa via wet mount preparation. Currently, diagnosis of the organism is by laboratory assays included in vitro culture and molecular methods. In this study, using by Polymerase Chain Reaction (PCR) method and based on Fe-hydrogenase gene amplification T. gallinae examined. This study carried out for investigation of Trichomonas contamination of pigeon of the clombidae family that included 2 Laughing Dove (Spilopelia senegalensis), 2 Rock Dove (Columba livia) and 2 Domestic Pigeon (Columba livia domestica). In this examination the samples were gathered from mouth and larynx of pigeons of Urmia by swab and immediately inculated in Trypticase Yeast extract Maltose medium (TYM medium). T. gallinae organisms in the logarithmic phase of growth in TYM medium used for specific PCR reaction using a primer pair of Fe-hydrogenase gene. The molecular results showed that PCR with primers Trich-1F and Trich-1R yielded 390-base pair band fragment. In addition, Using genomic DNAs of T. gallinae in pigeons of the clombidae family as a template, we obtained an amplification product of the same size (390bp) in all of pigeons and comparison of the Fe-hydrogenase gene showed no sequence variation between isolates.

Keywords:

Trichomonas gallinae , Molecular detection , Fe-hydrogenase gene , Columbidae birds , PCR