A coupled microbial fuel cell: using Shewanella and Bacillus subtilis with the potential of producing protease

Microbial fuel cells are devices that generate electricity by using the chemical energy which is hidden in the substrates. Bacillus subtilis and Shewanella, connected in series, were used in order to increase the voltage. A bio cathode was used as an oxygenacceptor which was filled by Chlorella vulgaris. An amount of 200 mL molasses was used in the anode, and 200 mL BBM in the cathode. Nafion 115 was used as a PEM at 27˚C under microaerophilicconditions prepared for these experiments. At the same time as producing electricity, the basic protease was generated in the anode chamber containing B.subtilis. This protease was detected byBradford’s test. The series connection of MFCs increased the voltage, and for the first time, we succeeded in producing biological products like enzymes in MFCs.

Keywords:

Bacillus subtilis , Bioenergy , Shewanella , Microbial Fuel Cell