Ziba Moghadasi - Department Of Marine Biology, Faculty Of Marine Sciences And Technologies, Science And Research Branch, Islamic Azad University.Tehran, Iran

Carpet anemone, Stichodactyla haddoni is an ocean dwelling sedentary organism belongs to thefamily of Stichodactylidae. These family contains specialized cells called nematocysts that producevenom for defense/offence purposes .These compounds is effective in medical applications andprevention of various diseases such as cancer. This study is survey of cytotoxicity of the methanolextract from Persian Gulf sea anemone, S. haddoni on Breast cancer (MD-MBA231) cell line by MTTassay. One sample of S. haddoni were collected from coastal waters of Lark island Persian Gulf thesouth of Iran in September 2015.The specimen after identification were kept in -20°C.The extractionof methanol from tentacle was performed and to put in store by freeze-drying. Amount of proteincontent was determined by bicinchoninic acid assay. The cell line was cultured in complete tissueculture medium RPMI+FBS10%+pen/strep. These cells were treatment in presence of differentSerial dilution 100 to 0.78 μg of venom extract in duration time 24 hour. The results of Lc50 showedthat after passing of 24 hour the death rate was 3.125 μg/ml. Analysis of variance for cytotoxicity ofcrude venom on MD-MBA231 cell line showed that activity was similar together in almost all doses(P <0.001). Linear regression analysis showed significant correlation between cytotoxicity of crudevenom on MD-MBA231 and examined doses (R2 = 0.692). This similarity would be suggested acommon dose dependent trend of lethality on cancer cell line. Therefore, further research aboutthese compounds is essential to can provide new biomedical research tools for designing novelanticancer drugs.