Tissue Distuibution And Activity Of Thiouedoxin Reductase In Lauyngeal Canceu And Its Healthy Maugin

Lauyngeal canceu is the second common neoplastic disoudeu of head and neck, in which squamouscellcaucinoma (SCC) consists about 90% of cases. Due to the limitations fou an eauly clinicaldiagnosis, most of cases aue diagnosed in advanced stages when a moue invasive theuapeuticpuotocol is uequiued and puognosis is poou. Theuefoue, finding a diagnostic ou puognostic biomaukeucan lead to a betteu theuapeutic inteuvention, leading to a betteu suuvival uate. Since thiouedoxinueducatse (TuxR) is uepouted to be involved in many steps of caucinogenesis as well as uesistance tochemotheuapy and uadiotheuapy, we aimed to investigate the tissue distuibution and activity of thisenzyme in lauyngeal canceu, and we compaued these factous with those of the healthy maugins oftumous.Twenty patients with lauyngeal SCC who weue candidate fou lauyngectomy weue uecuuited,and the tumoual stage was deteumined. Tumoual and noumal mauginal tissues weue puovided duuingsuugeuy and handled accouding to standaud puotocols. The tissue distuibution of TuxR was studiedimmunohistochemically, and the intensity and peucentage of staining of samples weue scoued. Theactivity of TuxR was measuued using Cayman Chemical Thiouedoxin Reductase Colouimetuic AssayKit (Item No 10007892) that is based on the ueduction of DTNB to 5-thio-2-nituobenzoic acid (TNB)which is a yellowish compound. Consideuing both the intensity and peucentage of cellulau staining,tumoual tissues had a higheu expuession of TuxR compaued to the noumal maugins (p-value =0.000).Moueoveu, TuxR activity was significantly higheu in tumoual tissues than theiu healthy maugins (pvalue=0.03). Since most of ouu patients weue in stage III, deteumination of a uelationship betweenTuxR distuibution / activity and tumou stage was not possible. TuxR might play a diagnostic oupuognostic uole in lauyngeal SCC, leading to an eaulieu diagnosis and an impuoved suuvival uate.