The association between rs2641726 single nucleotide polymorphism of muc4 gene related to hsa- mir-581 with gastric cancer incidence

Introduction: Gastric cancer is considered as the fourth most common cancer worldwide. MUC 4 is aberrantly expressed in gastric cancer. MUC4 can regulate cell apoptosis via both ErbB2 dependentand independent mechanisms. In this research, we aimed to evaluate the possible association between rs2641726 (C> A) polymorphism of MUC 4 and gastric cancer risk in the Iranian population. Materials& methods: In this case-control study, we enrolled 168 patients vs 66 healthy subjects. Allele-specific primer polymerase chain reaction (ASP-PCR) method was applied to genotype rs2641726 in patients with gastric cancer. Standard cycling was performed under the following conditions: Initial denaturation at 95 ºC for 5 min followed by 35 cycles of 94 ºC for 30 s, 55.6 ºC for 30 s, and 72 ºC for 50 s, and finally 72 ºC for 10 min. Results: According to the hardy Weinberg equilibrium, rs2641726 single nucleotide polymorphism are not associated with the gastric cancer incidence in Iranian population (P> 0.05) but we observed significant association between rs2641726 (C> A) polymorphism and control group (OR = 0.30 for A/C and C/C, P-value =<0.0001 and AIC = 265.9), Metastasis negative (OR = 0.27, P-value= 0.014 and AIC = 195.8), no Smoking (OR = 2.27, P-value =0.0004 and AIC = 92.7), H. pylori positive (OR = 2.05 for C/C, P-value =0.2 and AIC = 175.1) and Stage (Sig (Model Fitting Information) = 0.834). Conclusion: rs2641726 single nucleotide polymorphism of MUC4 gene related to hsa- miR-581 was not associated with gastric cancer incidence but some of histopathological features such as Metastasis negative, H. pylori positive, Stage and no Smoking have significant association with rs2641726 polymorphism

Keywords:

Gastric Cancer , Gene and Cancer , Cell and Cancer , Cancer Risks , Cancer Genetics