Potentiometric Determination of vancomyin using Ion- Selective Electrode

In this study‚ potentiometric determination of small amounts of vancomycin hydrochloride using ion-selective membrane electrode has been investigated. Design and preparation of electrode is based on PVC membrane‚Vancomycin- phosphomolibdic ion pair as the sensing element‚DBP as plasticizer and THF as solvent. The electrode exhibits a Nernestian response with a slope of about 56/02 mv in concentration range of 10-5 -10-2 M in pH range of 4-6. The low detection limit of the electrode is about 3×10-5 and the response time of about 15 s. The proposed electrode was used for at least 4 weeks without any measurable change in sensitivity. No interference was observed towards some inorganic ions and also some drugs. The electrode was successfully employed for the measurement of Vancomycin-Hydrochloride in the form of vial purchased from the local pharmacy and good recovery about %97 was obtained. With regards to the simplicity ‚speed‚accuracy‚sensitivity‚selectivity and good recovery this electrode can be used as a convenient and reliable tool for quantitative analysis of vancomycin.