Dendrosomal Nano-Curcumin is inducing apoptosis in MCF-7 and T47D breast cancer cell lines via downregulatingsurvivin expression

Breast cancer is one of the most common cancers among women but despite to the recent advances in cancer treatment, the lack of new treatment strategy with less side effect, is evident. On the other hand, common treatments have unwanted side effects. Nowadays refocusing on traditional medicine, not only for the safety of natural products but because of their rich complex chemicals with anticancer effects, is impressive.However, hydrophobic nature of phytochemicals prevent their absorption. Recent researches show that new form of curcumin called Dendrosomal Nano-Curcumin (DNC) has more anticancer effects and induces the apoptosis much more effective in compare to its free form. Therefore, in this study we have evaluated anticancer effect of DNC on down-regulating survivin and its variants as an anti-apoptotic oncogene in MCF-7 and T47D cell lines. For this aim, toxicity effect of DNC (IC50) was estimated using MTT assay and respectively cells were treated intime and dose titration. Then, the expression of survivin and its variants evaluated using Real-Time PCR technique. Primary results suggest significant decreases in survivin and its variants expression. Overall, our results confirm the strong apoptotic effect of DNC which is associated with significant decrease in antiapoptotic activity of Survivin

Keywords:

Breast cancer , Dendrosomal Nano-Curcumin , apoptosis , survivin , MCF-7 , T47D