Afsane Soleimani - Department of Biochemistry and Biophysics, Disorder Metabolic Research Center, Faculty of Medicine, Golestan University of Medical Sciences, Iran
Faramarz Rostami-Charati - Department of Chemistry, Faculty of Science, Gonbad Kavous University, P.O. Box ۱۶۳, Gonbad, Iran
Jahanbakhsh Asadi۱ - Department of Biochemistry and Biophysics, Disorder Metabolic Research Center, Faculty of Medicine, Golestan University of Medical Sciences, Iran

Background: Benzofuran compounds display diverse array of pharmacological activities among anticancer. This study was focused on evaluation of the cytotoxicity and apoptotic affects of benzofuran derivative on MCF-7 breast cancer cell line. This effective compound was isolated from the root of Petasites hybridus plant. Methods: For experiments, the MCF-7 cells were treated with several concentrations (0-500μM) of benzofuran compound at different times. In this study, test of neutral red was also employed for cytotoxicity assay and quantity of P53, P21. Bax genes expression was analyzed using Real-Time PCR and ELISA techniques. Results and conclusion: Results show that compound 1 has cytotoxicity and apoptotic effects on Human breast cancer cells. Further studies are needed to determine the mechanism of action of this compound and For definitive conclusion, further studies is essential on other cancer cell lines, non-tumor cells and animals models and subsequent clinical studies.

Apoptosis, benzofuran derivative, Cytotoxicity, MCF-7 breast cancer cell line, petasites hybridus