Effect of PAI-1 4G/5G polymorphism in breast cancer patients from North West of Iran

Introduction: Among the variants of the PAI-1 gene, the 4G/5G polymorphism has been the most frequently studied, because its location at the promoter of the gene indicates its possible role in the regulation of PAI-1 transcription. Therefore, the aim of this study was to investigate the effect of this polymorphism in patients with breast cancer from North West of Iran. Methods: 200 breast cancer patients and 200 healthy controls from North West of Iran were selected. Peripheral blood-DNA samples were used for PCR amplification using the allele specific primers. The collected data were analyzed through using Fisher’s exact tests and SPSS software, with a significance level of 0.05. Results: Based on molecular studies, all patients and control group were divided into three genotypes 4G/4G, 4G/5G and 5G/5G. Genotype distribution and alleles frequencies between patients and control group were calculated. Discussion and conclusion: Results indicate that the genotypic and allelic frequencies of PAI-1 4G/5G polymorphism showed no significant difference between breast cancer patients and control individuals in this cohort.

Keywords:

PAI-1 4G/5G polymorphism , breast cancer , North West of Iran