Study of antibiotic resistance in Pseudomonas species isolated from clinical samples of Kerman Hospitals by Multiplex PCR

Pseudomonas aeruginosa is one of the most common human pathogen. It is the causative agent for 10% of nos-ocomial infections. Accordingly, Identification of these bacteria helps public health programs in the shortest time. With the rapid detection of virulence genes related to these bacteria and enhancing our information abot resis-tant genes in each area, treatment of patients can be done in a better way. So, by evolution of our information about virulence gene as well as specific detection of bacteria, treatment of patients can be done more efficiently and on time In this study,12Pseudomonas aeruginosa strains were isolated from patients in Kerman hospitals from various sources of clinical wounds caused by burns. By Multiplex PCR assay and specific primers, antibiotic resistant genes qnrAM, qnrBM, qnrSM were identified among test bacteria. According to Multiplex PCR samples, 0 of 0% of qnrA positive, 2 samples (16/66%) of qnrB positive and 2 samples (16/66%) qnrS were positive. Rapid detection and accurate reporting of these bacterial strains in hospitals can lead to better control and more effec-tive dissemination of these strains. The use of molecular methods for diagnosis of resistant genes in pathogenic bacteria including hospital-acquired infections such as Pseudomonas aeruginosa could be helpful to know the epidemiology of resistant bacteria and to find a better way to defend by suitable drugs

Keywords:

Pseudomonas aeruginosa , nosocomial infections , Multiplex PCR , qnr genes