Armin Attaranzadeh - Fertility Research Centre, Milad Hospital, Mashhad,Iran
Mahshid Salehabadi - Department of Biochemistry, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Iraj Khodadadi - Department of Biochemistry, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Marzieh Farimani - Fertility Research Centre, Fatemieh Hospital, Hamadan, Iran

Background: PCOS is one of the most common endocrine disorders occurring in women. Epidemiological studies have resulted in estimates of prevalence, in Iranian women of reproductive age at14.6% using biochemical and/or clinical evidence. The correlation between the activity of adenosine deaminase, which deaminates adenosine and 2-deoxyadenosine, and the follicular growth and development has previously been documented. However there is no information available about the impact of adenosine deaminase polymorphisms on polycystic ovary syndrome (PCOS). Therefore, this study aimed to evaluate the enzymatic activity of total adenosine deaminase as well as its isoenzyms, ADA1 and ADA2, in women with PCOS and healthy women groups.Methods: Two hundred women with PCOS and 200 healthy female subjects were enrolled in this paired-match study. The serum activity of adenosine deaminase was measured by Giusti and Galanti colorimetric method.Result: tADA activity was significantly lower in PCOS compared with control (27.76±6.0 vs 39.63±7.48, respectively). PCOS patients also showed reduced activity of ADA1 and ADA2.Conclusion: The present study showed that women with PCOS remarkably reduced activities of total adenosine deaminase and its ADA1 and ADA2 isoenzymes compared with health women. Therefore, it can be concluded that adenosine may play an important role in the development and progression of PCOS.

ADA1 isoenzyme, ADA2 isoenzyme, Polycystic ovary syndrome, Total adenosine deaminase, Adenosine deaminase